Ramisa Fariha

Electric field-assisted dried blood spot sample preparation for analysis of steroids using LC–MS/MS

Despite being a minimally invasive sample source, dried blood spots (DBS) generally pose the challenge of efficient sample extraction for broad metabolomics applications. This is particularly true for the quantification of steroids using non-derivatized liquid chromatography tandem mass spectrometry assays. To address these limitations, we have demonstrated the use of electric field as a driver for sample preparation from DBS samples to simultaneously quantify testosterone (T), 17α-hydr....

An in-depth analysis of four classes of antidepressants quantification from human serum using LC–MS/MS

Depression is a growing global crisis, with females at a higher rate of diagnosis than males. While the percentage of patients on prescribed antidepressants have tripled over the last two decades, we are still at a crossroad where the discrepancy lies between finding a drug to suit a patient and monitoring the abundance of it in the body to prevent unwanted side effects. Liquid Chromatography tandem mass spectrometry (LC–MS/MS) has garnered the attention of clinicians as a technique to....

Simultaneous detection of salivary cortisol and cortisone using an automated high-throughput sample preparation method for LC-MS/MS

High-Performance Liquid Chromatography-Tandem Mass Spectrometry has emerged triumphant over the years as a reliable and high throughput clinical instrument to assess different metabolic irregularities. One of the latest applications of LC-MS/MS has been in the field of quantification of glucocorticoids, such as cortisol and cortisone from saliva, that can be an indicator of abnormalities such as Congenital Adrenal Hyperplasia (CAH), Cushing's Syndrome, and Addison's disease. We have d....

High Throughput Low-Level Testosterone detection in Human Serum using a stand-alone LC-MS/MS Assay with Protein Precipitation

The measurement of free testosterone (T) in human serum at very low levels is pivotal in the clinical practice of diagnostics, treatment, and prevention of a variety of sex-hormone related disorders. This includes the diagnosis of hypogonadism and androgen deficiencies in men and polycystic ovarian syndrome (PCOS) in women, as well as various types of cancers. Moreover, abnormally excessive or deficient T levels in patients may be associated with doses and side effects of patient medications....

Research

Electric field-assisted dried blood spot sample preparation for analysis of steroids using LC–MS/MS

An in-depth analysis of four classes of antidepressants quantification from human serum using LC–MS/MS

Simultaneous detection of salivary cortisol and cortisone using an automated high-throughput sample preparation method for LC-MS/MS

High Throughput Low-Level Testosterone detection in Human Serum using a stand-alone LC-MS/MS Assay with Protein Precipitation

Simultaneous detection of Salivary Cortisol and Cortisone using a High-throughput Sample Preparation for LC-MS/MS

SimplicityLabCR: An Integrated Liquid-Handling LC-MS/MS Platform for Therapeutic Drug Monitoring

Establishing Optical Coherence Tomography (OCT) as an EffectiveMethod for Investigating Cellular Viability in 3D Breast Cancer Spheroids

The Effects of Processing on Urinary Bladder Matrix (UBM) Characteristics

A Comparison of the Composition and In Vitro Macrophage Response between MicroMatrix® and AmnioFill®